Substrate specificity of cholesterol oxidase.
نویسندگان
چکیده
منابع مشابه
Xanthine Oxidase. Vi. Influence of Ph on Substrate Specificity.
Milk xanthine oxidase exhibits a broad substrate specificity that includes purines, aldehydes, and pteridines (1). The mechanism of substrate oxidation, particularly that of purines, has been the subject of several studies (2-4). The present report concerns the observation of a new class of substrates, quaternary heterocyclic compounds, which are oxidized at pH values above 9.6. A partial list ...
متن کاملEngineering the substrate specificity of D-amino-acid oxidase.
The high resolution crystal structure of D-amino-acid oxidase (DAAO) from the yeast Rhodotorula gracilis provided us with the tool to engineer the substrate specificity of this flavo-oxidase. DAAO catalyzes the oxidative deamination of D-amino acids, with the exception of D-aspartate and D-glutamate (which are oxidized by D-aspartate oxidase, DASPO). Following sequence homology, molecular model...
متن کاملPlant sterol and stanol substrate specificity of pancreatic cholesterol esterase.
Consumption of plant sterols or stanols (collectively referred to as phytosterols) and their esters results in decreased low-density lipoprotein cholesterol, which is associated with decreased atherosclerotic risk. The mechanisms by which phytosterols impart their effects, however, are incompletely characterized. The objective of the present study is to determine if pancreatic cholesterol ester...
متن کاملStudies of the substrate specificity of cholesterol oxidase from Nocardia erythropolis in the oxidation of 3-hydroxy steroids.
going reduction. However, the carbonyl group of the larger, and more polar, 178-0(3-carboxypropanoyl)glycollyl side chain was not reduced. A 17/3-carboxyl or 178-methoxycarbonyl side chain was not reduced, probably for electronic reasons. In this respect, the reducing power of cortisone reductase resembled that of NaBH4 or lithium tri-t-butoxyaluminium hydride, and was less than that of LiAIH4....
متن کاملSTREPTOMYCES FRADIAE, A POTENT PRODUCER OF CHOLESTEROL OXIDASE
Thirteen species of Streptomycetes, Arthrobacters and Mycobacterium were studied for the determination of a potential cholesterol oxidase producer. Only Streptomyces fradiae and Streptomyces rishiriences were found to be capable of producing this enzyme. Streptomyces fradiae was selected for the production of cholesterol oxidase and cultured in different media to find optimum media and con...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1980
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/26.5.678